P33. NK-92 cellular immunotherapy as an alternative to donor derived peripheral blood NK cells
نویسنده
چکیده
Methods Collecting NK cells form donors requires them to undergo leukapheresis with subsequent removal of CD3+ lymphocytes (to prevent GvHD) and expansion and activation of the NK cell enriched fraction with IL-2. In contrast, the continuously growing NK cell line NK-92 can be easily expanded to clinical scale in bioreactors. The broad cytotoxicity of NK-92 is due to the lack of most of the KIR receptors while expressing a range of activating receptors.
منابع مشابه
جدا سازی و تخلیص زیر مجموعه های سلول های کشنده طبیعی با استفاده از مایع رویی کشت سلول های تک هسته ای به کمک PHA و ارزیابی قدرت کشندگی آنها
Natural Killer (NK) cells are the main lymphocyte population expressing P75 B chain of the IL-2 receptor (IL-2R). Consequently, incubation of peripheral blood lymphocytes with IL-2 induce selective activation of NK cells and results in NK activity and generation of Lymphokine activated killer (LAK) cells activity and proliferation. One of the early events during IL-2 activation of peripheral bl...
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